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Q: Fachverband Quantenoptik und Photonik

Q 41: Nano-Optics

Q 41.17: Poster

Wednesday, March 16, 2022, 16:30–18:30, P

Flat-top beam shaping and it's use in modern microscopy — •Leona Licht¹, Philipp Kellner¹, Giovanni DeAngelis¹, Christian Eggeling^1,2, and Herbert Gross³ — ¹Institut für angwandte Optik und Biophysik, Friedrich-Schiller-Universität, Philosophenweg 7, 07743 Jena — ²Leibnitz-Institut für photonische Technologien, Albert-Einstein-Straße 9, 07745 Jena — ³Institut für angwandte Physik, Friedrich-Schiller-Universität, Albert-EInstein-Straße 15, 07745 Jena

Light microscopy, although known for more then 300 years by now, is still one of the most versatile tools for observation of living biological samples and their analysis. Nowadays illumination in most microscopes is done by laser-radiation and it's inherent gaussian profile. On this poster we present recent developments in microscope illumination using flat-top shaped laser-beams paving the way towards highly uniform illumination used in single-molecule tracking or as a prerequisite in advanced analysis methods. The beam shaping can be achieved by microstructured phaseplates or by special optical components. We will elaborate on the usability of these all-optics approaches to beam-shaping in widefield and confocal microscope configurations.