Regensburg 2022 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 12: Poster 2
BP 12.1: Poster
Dienstag, 6. September 2022, 17:30–19:30, P4
Holographic vibration spectroscopy: Probe- and contact-free viscoelastic analysis of adherent cells — •Bob Fregin1,2, Stefanie Spiegler1,2, and Oliver Otto1,2 — 1ZIK HIKE, University of Greifswald, Greifswald, Germany — 2DZHK, University Medicine Greifswald, Greifswald, Germany
Cell mechanical properties can be used as an inherent biomarker for cell state, fate and function. Several high-throughput methods are available to characterize suspension cells, e.g., peripheral blood cells, without any labeling. However, fast and robust methods are lacking for adherent cells, although the majority of cells, e.g., in our human body, is aggregated into tissues.
Here, we introduce a new probe- and contact-free method for label-free mechanical phenotyping of adherent cells at high spatiotemporal resolution. While cells are excited mechanically by a vibration in the range of 100 kHz, their response is determined optically from cell height oscillations utilizing holographic laser Doppler interferometry. In proof-of-concept experiments on a monolayer of induced pluripotent stem cells (iPSCs), we present a cell amplitude response as a function of varying excitation amplitudes. This amplitude response is proportional to the elastic properties of a cell.
In future work, we plan to perform a spectroscopic evaluation, where experiments are carried out at multiple frequencies. Further, we aim to extend our analysis to a complete viscoelastic description.