Regensburg 2022 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 12: Poster 2

BP 12.10: Poster

Dienstag, 6. September 2022, 17:30–19:30, P4

Mechanical fingerprint of the intra-cellular space — •Till M Muenker and Timo Betz — University of Goettingen, Goettingen, Germany

Many important cellular functions such as organelle positioning and internal cargo transport are dependent on the viscoelastic intracellular mechanical properties of cells. A range of different mechanical models has been proposed to describe these properties. Whilst simple models such as Maxwell or Kelvin-Voigt models don't seem sufficient to capture the full complexity of cells, more elaborate models like generalized Kelvin-Voigt models require a huge number of parameters. This hinders the comparison and interpretation of experimental findings. Further, from a physics perspective, cells are systems out of thermodynamic equilibrium, permanently consuming metabolic energy to carry out mechanical work. The level of "non-equilibrium" can be proposed as an indicator for cell type, cell state or even diseases. To determine both, the viscoelastic properties and the cellular activity, we use optical tweezers based active and passive microrheology in a diverse group of 9 different cell-types. Surprisingly, despite differences in origin and function, the complex moduli of all cell types can be described using a 4 parameter based fractional Kelvin-Voigt model. Additionally, the frequency dependent activity can be described with a simple power law. This approach allows to reduce those complex and frequency dependent properties down to a fingerprint of 6 parameter. Further principal component analysis shows that only 2 of them may be sufficient to characterize the mechanical intracellular state.