Regensburg 2022 – wissenschaftliches Programm

Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe

BP: Fachverband Biologische Physik

BP 12: Poster 2

BP 12.12: Poster

Dienstag, 6. September 2022, 17:30–19:30, P4

Optimization of patterned polyacrylamide gels for traction force microscopy — •Ina Braun, Mohammad Armin Eskandari, Fatemeh Abassi, and Timo Betz — Third Institute, Biophysics, Georg August Universität, Göttingen, Germany

Combining micropatterned adhesion with soft polyacrylamide gels is widely described in literature, however the practical experience shows a series of possible artifacts. The problems are typically a variation of fluorescent bead localization in response to the ECM proteins applied. In detail we find changes in the bead distribution that we aim to understand and avoid. Micropatterns of various ECM proteins are initially created on glass coverslips using a photomask. Subsequently, they are transferred on the polyacrylamide gels containing fluorescent beads during the polymerization process. In an additional step we compare the classical protocol of pattern tranfer during polymerization with a more specific approach by including NHS-acrylamide in the hydrogel premix. After pattern transfer we quantify the bead localization, homogeneity and potential clustering at the pattern sites with the non-patterned regions. We optimize the bead distribution by systematic variation of pH value and ion composition of the premix. The potential of cell adhesion and traction force microscopy is assed in the final step.