Regensburg 2022 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
BP: Fachverband Biologische Physik
BP 12: Poster 2
BP 12.29: Poster
Dienstag, 6. September 2022, 17:30–19:30, P4
Local organization of F-actin studied via Förster resonance energy transfer using 2D polarization fluorescence imaging (2DPOLIM) — •Mohammad Soltaninzehad1,2, Rainer Heintzmann1,2, Adrian T. Press3,4, and Daniela Täuber1,2 — 1Leibniz Institute of Photonic Technology, Jena — 2Institute of Physical Chemistry & Abbe Center of Photonics, Friedrich-Schiller-University Jena, Germany — 3Department of Anesthesiology and Intensive Care Medicine, Jena University Hospital — 4Faculty of Medicine, Friedrich Schiller University Jena, Germany
2D polarization fluorescence imaging (2DPOLIM) provides complete in-plane evaluation of the polarization state of the sample[1,2], giving access to macromolecular arrangement in the range of 2-10 nm via Förster resonance energy transfer between similar fluorophores (homo-FRET, emFRET). Phalloidin-dye complexes map the structure of F-Actin, by binding specifically. We applied 2DPOLIM to phalloidin-DY490 stained liver tissue of mice from different treatment groups in the context of polymicrobial sepsis[1,3]. Qualitative analysis showed significant differences in the molecular arrangement of F-actin in agreement with the survival of the animals. Further information will be obtained from comparing the experimental data to a series of simulations[2]. -- Funding by DAAD-GSSP, DFG-Ta1049/2, Interdisziplinäre Zentrum für Klinische Forschung Jena (AMSP-05). -- [1] D. Täuber et al. ELMI 2021, https://doi.org/10.22443/RMS.ELMI2021.6. [2] R. Camacho et al. Commun. Biol. 2018, 1, 157. [3] A.T. Press et al. EMBO Mol. Med. 2021, 13 (10), e14436.