Regensburg 2022 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
BP: Fachverband Biologische Physik
BP 12: Poster 2
BP 12.41: Poster
Dienstag, 6. September 2022, 17:30–19:30, P4
Single Cell Force Spectroscopy: The Impact of Cell Contact Area — •Sophie Geiger1, Maria Villiou1,2,3, and Christine Selhuber-Unkel1,2,3 — 1Institute for Molecular Systems Engineering, University of Heidelberg, DE — 2Max Planck Schools: Matter to Life, University of Heidelberg, DE — 3Cluster of Excellence 3DMM2O KIT & Heidelberg University, DE
Single cell force spectroscopy is a versatile method for characterising cell-substrate interactions. It has already been applied in several studies to investigate the effect of photomechanical stimulation and the influence of structuring molecules on cell detachment forces.
A critical aspect is the contact area between cell and substrate, as a larger contact area usually leads to higher cell detachment forces. However, the contact area between cell and substrate varies with cell size and with the deformation of the cell pressed onto the surface.
We aim to avoid the distortion that these variations exert on the cell detachment forces by limiting the adhesion area. This is achieved using micropatterned substrates. We use light-induced molecular adsorption of proteins (LIMAP) to generate circular fibronectin micropatterns on an inert background. In a systematic study, we investigate the dependence of cell detachment forces on the cell-substrate interaction surface.