Regensburg 2022 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 13: Cytoskeleton

BP 13.9: Vortrag

Mittwoch, 7. September 2022, 12:00–12:15, H15

Correlative Super-Resolution Microscopy and Structural Analysis of Cells and Tissues — •Dimitar Stamov, Tanja Neumann, André Körnig, Torsten Müller, and Heiko Haschke — JPK BioAFM, Bruker Nano GmbH, Am Studio 2D, 12489 Berlin, Germany

Active forces in biological systems define the interactions between single molecules, growing cells and developing tissues. Cells adapt their shape and react to the surrounding environment by a dynamic reorganization of the F-actin cytoskeleton. We will demonstrate how cell spreading and migration in living KPG-7 fibroblasts and CHO cells, can be studied with high-speed AFM and associated with spatially resolved cytoskeletal reorganization events. We will further extend this with high-speed mechanical mapping of confluent cell layers, which in combination with optical tiling can be applied to automated analysis of large sample areas. We will show how AFM imaging and super-resolution 2color easy3D STED measurements can be combined and will show results of co-localized imaging and sample manipulation with a precision below the diffraction limit. We will discuss how to calculate the viscoelastic properties, characterized by the dynamic storage and loss modulus distribution in such samples.