Regensburg 2022 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 26: Focus Session: Bioinspired Systems
BP 26.7: Vortrag
Donnerstag, 8. September 2022, 17:00–17:15, H15
New insights into the DNA origami silicification reaction mechanism by in situ small angle X-ray scattering — •Amelie Heuer-Jungemann1,3, Martina Ober2, Lea Wassermann1, Anna Baptist1, and Bert Nickel2,3 — 1Max Planck Institut für Biochemie, Am Klopferspitz 18, 82152 Martinsried — 2Ludwig-Maximilians-Universität, Geschwister-Scholl-Platz 1, 80539 München — 3Center for Nanoscience, LMU München, Geschwister-Scholl-Platz 1, 80539 München
DNA origami allows for the formation of arbitrarily shaped nanostructures with nm precision control. Yet, many potential real-life applications have been hampered due to the biologicL instability of DNA origami: Silicification provides an excellent way of increasing DNA origami stability. However, so far, it remains unclear how silicification affects the internal structure of the DNA origami and whether the whole DNA framework is embedded or if silica just forms an outer shell. By using in situ small angle x-ray scattering (SAXS), we were able to show that silica growth is not restricted to the outer origami surface, but also occurs on the inner surface, penetrating the whole structure and induces substantial condensation of the structure at early reaction times. Remarkably, we found that thermal stabilization of the origami up to 60°C as well as resistance towards degradation by nucleases could already be observed for sub-nm silica deposition in the highly condensed state. In this state DNA origami addressability could also be retained, resulting in the first fully site-specifically addressable silica nanostructure.