Regensburg 2022 – wissenschaftliches Programm

Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe

BP: Fachverband Biologische Physik

BP 5: Focus Session: Super Resolution Microscopy and Dynamics of Supramolecular Complexes

BP 5.5: Vortrag

Montag, 5. September 2022, 16:45–17:00, H15

Spatiotemporal SARS-CoV-2 binding dynamics investigated with 100 Hz ROCS microscopy and thermal fluctuation analysis — •Dominik Huber and Alexander Rohrbach — Laboratory for Bio- and Nanophotonics, Department of Microsystems Engineering - IMTEK, University of Freiburg, 79110 Freiburg, Germany

The emergence of the new severe acute respiratory syndrome corona virus 2 (SARS-CoV-2) in recent years has caused tremendous interest in investigating the interactions between viruses and cells. Especially the imaging and tracking of viruses are of great importance to see and understand the binding and uptake of viruses into cells and to be able to intervene in these processes. Due to their small size and fast movement imaging virus dynamics is a very challenging task, which has so far been achieved with different fluorescence methods, being limited by photo bleaching and imaging speed.

In our research we apply Rotating Coherent Scattering (ROCS) microscopy in order to visualize the diffusion of SARS-CoV-2 virus like particles (VLPs) close to A549 lung epithelial cells. ROCS microscopy allows for label-free imaging with more than 100 Hz temporal at 150 nm spatial resolution. The high contrast image stacks enable single particle tracking and characterization of the binding process and strength of VLPs with SARS-CoV-2 spike proteins to A549 cells using thermal fluctuation analysis.