Regensburg 2022 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 7: Poster 1
BP 7.33: Poster
Montag, 5. September 2022, 18:00–20:00, P1
Fixed 4-channel detection in 2D polarization fluorescence imaging (2DPOLIM) and compensation of depolarization caused by dichroic mirrors — •Yutong Wang1,2, Asad Hafeez1,2, Dijo Moonnukandathil Joseph1,2, Mohammad Soltaninezhad1,2, Rainer Heintzmann1,2, and Daniela Täuber1,2 — 1Leibniz Institute of Photonic Technology — 2Friedrich-Schiller-University Jena, Germany
Polarization resolved fluorescence imaging (POLIM) can reveal macromolecular structure in the range of 2-10 nm via Förster resonance energy transfer between similar fluorophores (homo-FRET, emFRET). 2D POLIM is superior to conventional fluorescence anisotropy methods for studies of anisotropic samples[1,2]. Implementing a variable electrooptic polarization control in the excitation together with a fixed 4-channel detection[3] speeds up the acquisition giving access to polarization resolved snapshots of dynamic samples. A major issue in POLIM setups is depolarization caused by the multilayer coating of dichroic mirrors, which introduces phase shifts between s- and p-polarized components. We implemented two pairs of dichroics with orientations crossed to each other. By this, incident s- and p-polarization is exchanged within each pair. The design proved to be an effective and reliable approach to significantly improve the quality of polarization by compensating the depolarization introduced by a single dichroic. -- Funding: DFG-Ta1049/2 -- [1] R. Camacho et al. Adv. Mater. 2019, 1805671. [2] R. Camacho et al. Commun. Biol. 2018, 1, 157. [3] F. Zimmermann et al. in Optically Induced Nanostructures, 2015.