Regensburg 2010 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 29: Biomolecular Spectroscopy
BP 29.3: Vortrag
Donnerstag, 25. März 2010, 15:00–15:15, H45
Characterization of artificial peptide receptors by UV resonance Raman spectroscopy and non-negative matrix factorization — •Christoph Herrmann1, Stephan Niebling1, Sunil Kumar Srivastava1, Carsten Schmuck2, and Sebastian Schlücker1 — 1Fachbereich Physik, Universität Osnabrück, Barbarastr. 7, 49069 Osnabrück — 2Institut für Organische Chemie, Universität Duisburg-Essen, Universitätsstr. 5, 45141 Essen
Guanidiniocarbonyl pyrroles are artificial peptide receptors which serve as model systems for investigating the principles of peptide binding. Their carboxylate binding site (CBS) can be selectively monitored by UV resonance Raman (UVRR) spectroscopy. UVRR spectra of guanidiniocarbonyl pyrroles we recorded at different pH values in the range of 4 to 9 in order to characterize the corresponding acid/base equilibrium. By using non-negative matrix factorization (NMF), we were able to extract the pure spectra of the neutral and protonated CBS species from the experimental UVRR spectra. This allowed the quantification of their relative contributions and the site specific pKa determination of the CBS in these artificial peptide receptors.