Regensburg 2010 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 5: Posters: Biopolymers and Biomaterials
BP 5.1: Poster
Montag, 22. März 2010, 17:15–20:00, Poster B1
Interactions between proteins and thermoresponsive microgels — •Nicole Welsch1 and Matthias Ballauff1, 2 — 1Soft Matter and Functional Materials, Helmholtz-Zentrum Berlin für Materialien und Energie GmbH, 14109 Berlin — 2Department of Physics, Humboldt University Berlin, 12489 Berlin
The interface of materials science and biology is emerging as a major research focus and especially nanomaterials in medicine and biotechnology are considered to accelerate the progress in these fields. However, nanoparticles entering the bloodstream initially become coated with proteins and the contact to these particles may induce misfolding of the protein structure and the perturbation of the function of the bound proteins. Therefore, this *nano-bio* interface has to be understood in detail for new applications to evolve. Particles based on poly(N-isopropylacrylamide) (PNiPA) exhibit lower critical solution behaviour close to the physiological temperature and therefore have potential to be applied in biotechnology. For deeper insight into the interactions of biomolecules to PNiPA microgels the adsorption of proteins with different surface properties is investigated. Thereby, SAXS experiments elucidate the spatial distribution of the proteins within the polymer network. Furthermore, the activity of immobilized enzymes is investigated temperature-dependent to analyse the impact of the immobilization on the catalytic activity. By using FT-IR spectroscopy changes of the secondary structure of adsorbed proteins and the formation of interactions towards the carrier particles can be analysed.