Regensburg 2010 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 6: Posters: DNA and DNA Enzymes

BP 6.10: Poster

Montag, 22. März 2010, 17:15–20:00, Poster B1

Towards sub second imaging of DNA compaction by AFM — •Jan Knappe¹, Sebastian Hanke¹, Szabolcs Sörös², Christoph F. Schmidt¹, Wolfgang Fischle², and Iwan A. T. Schaap¹ — ¹III. Physkalisches Institut, Georg-August-Universität, 37077 Göttingen — ²Max Planck Institute for Biophysical Chemistry, 37077 Göttingen

To compact and organize DNA the eukaryotic cell has developed several packaging steps. DNA is wrapped around histones to form nucleosomes, which in turn can be linked by other proteins to form larger aggregates. To study this system on a single molecule level we have set out to use atomic force microscopy (AFM) in liquid to image the binding and unbinding of DNA to the histone octamer. However to be able to study the dynamics of nucleosomal DNA on a biological relevant time scale, we need techniques that permit image acquisition in a second or faster. We will present our first results that combine imaging at low forces (~100 pN) with a high temporal resolution.