DPG Phi
Verhandlungen
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DPG

Regensburg 2010 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 6: Posters: DNA and DNA Enzymes

BP 6.4: Poster

Montag, 22. März 2010, 17:15–20:00, Poster B1

single-molecule force spectroscopy on phoB-DNA complexes — •Michael Birlo1, Katrin Wollschläger2, Rainer Eckel3, Norbert Sewald4, and Dario Anselmetti51Department of Physics Bielefeld University 33615 Bielefeld (Germany) — 2Department of Chemistry Bielefeld University 33615 Bielefeld (Germany) — 3Department of Physics Bielefeld University 33615 Bielefeld (Germany) — 4Department of Chemistry Bielefeld University 33615 Bielefeld (Germany) — 5Department of Physics Bielefeld University 33615 Bielefeld (Germany)

Interactions between proteins and DNA are essential for the regulation of cellular processes in all living organisms. In this context, it is of special interest to investigate the sequence-specific molecular recognition between transcription factors and their cognate DNA sequences. As a model system, peptide and protein epitopes of the DNA-binding domain (DBD) of the transcription factor PhoB from Escherichia coli are analyzed with respect to DNA binding at the single-molecule level. Quantitative AFM-DFS analysis proves the specificity of the interaction and yields force-related properties and kinetic data, such as thermal dissociation rate constants. An alanine scan for strategic residues in both peptide and protein sequences is performed to reveal the contributions of single amino acid residues to the molecular-recognition process.

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