Kiel 2011 – wissenschaftliches Programm
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SYMP: Symposium Interdisziplinarität Plasma + Medizin / Biologie
SYMP 1: Interdisziplinarität Plasma + Medizin / Biologie
SYMP 1.3: Hauptvortrag
Donnerstag, 31. März 2011, 15:00–15:30, HS H
Effects of reactive oxygen species (ROS) from cold plasmas on cellular activities — •Kai Masur1, Kristian Wende1, Manuela Harms2, Stephan Reuter1, Annemarie Barton1, Lena Bundscherer1, Sybille Hasse1, and Klaus-Dieter Weltmann1 — 1Leibniz Institut für Plasmaforschung und Technologie e.V. (INP) — 2Ernst-Moritz-Arndt Universität Greifswald Institut für Pharmazie
Due to the progress in recent years in the development and research of non equilibrium atmospheric pressure plasmas with high reactive species densities at low gas temperature, plasma treatment of living tissue has become possible and promises an efficient and improved treatment of infected or chronic wounds. Even treatment of formerly incurable skin diseases has come within reach. In plasma-cell/tissue interaction secondary effects play a crucial role. Cells require a liquid surrounding in order to survive * this means that plasma always influences the cell via the extracellular physiological environment acting as the interface. The effect of reactive oxygen species on cellular DNA of either an Argon or Air plasma (dielectric barrier discharge or an plasma jet) treated keratinocytes and fibroblasts is investigated by determining the ROS accumulation by oxidation of H2DCFDA. Subsequently the ROS effect on the integrity of the cellular DNA is measured by single gel electrophoresis (comet assay). Furthermore, cellular reactions such as apoptosis / necrosis versus proliferation have been investigated via flow cytometry and several electrophoresis based techniques. Special focus was put on cellular adhesions - analyzed by 3D time-lapse video microscopy and atomic force microscopy.