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BP: Fachverband Biologische Physik
BP 16: Poster IV
BP 16.14: Poster
Dienstag, 17. März 2020, 14:00–16:00, P2/EG
Imaging protein-based artificial molecular motors — •Ivan Unksov1, Pradheebha Surendiran1, Chapin Korosec2, Peter Jönsson3, Roman Lyttleton1, Damiano Verardo1, Roberta Davies4, Till Böcking5, Nancy Forde2, Paul Curmi4, and Heiner Linke1 — 1Solid State Physics and NanoLund, Lund University, Lund, Sweden — 2Department of Physics, Simon Fraser University, Burnaby, British Columbia, Canada — 3Department of Chemistry, Lund University, Lund, Sweden — 4School of Physics, University of New South Wales, Sydney, Australia — 5Single Molecule Science and ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales, Sydney, Australia
We are working on two concepts of artificial molecular motors: the Tumbleweed, a protein motor built from DNA-binding protein repressors (Bromley et al. HFSP J 2009), and the Lawnmower, a motor based on a microbead decorated with trypsin protease (Kovacic et al. IEEE Trans Nanobioscience 2015). The Tumbleweed is designed to make steps as small as 10 nm along a DNA upon switching of buffers which enable ligand-specific binding of motor to DNA. We show the binding using silica beads with attached multiple DNA tracks: we add motors with fluorescent labels and track the changes in the intensity and radius of fluorescence patterns; with this approach, we aim at seeing motor motion with sub-diffraction precision. For the Lawnmower, using fluorogenic peptides as the surface-bound substrate, we demonstrate the substrate cleavage by trypsin, which is expected to allow for the motion of motor based on rectified diffusion.