Dresden 2020 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 9: Poster II

BP 9.24: Poster

Montag, 16. März 2020, 17:30–19:30, P2/2OG

Ellipsometric study of DPPC Supported Lipid bilayer formation evaporated by a solvent-free process on silicon substratesMarcelo A. Cisternas1, Francisca Palacios-Coddou1, Sebastian Molina1, Maria Jose Retamal2, Nicolas Moraga1, Hugo Zelada1, Marco A. Soto-Arriaza2, Tomas P. Corrales3, and •Ulrich G. Volkmann11Institute of Physics and CIEN-UC, P. Univ. Catolica de Chile, Santiago, Chile — 2Faculty of Chemistry and CIEN-UC, P. Univ. Catolica de Chile, Santiago, Chile — 3Department of Physics, UTFSM, Valparaiso, Chile

Recent interdisciplinary studies of biological molecules with physical techniques have opened an emerging field for applications in bionanotechnology. The new techniques of preparation and characterization of supported lipid bilayers (SLB) contribute to the creation of new silicon-based nanodevices, which are an important input to the field of bionanotechnology. In this research, we report the novel formation process of supported Dipalmitoylphosphatidylcholine (DPPC) bilayers evaporated directly on bare silicon surfaces by means of physical vapor deposition, without the use of a polymer cushions or solvents, i.e. in a completely dry process. High-resolution ellipsometry measurements in air, without further hydration, detect the characteristic phase transitions of DPPC bilayers: gel to ripple 311.5 +/- 0.9 K, ripple to liquid crystalline 323.8 +/- 2.5 K, liquid crystalline to fluid disordered 330.4 +/- 0.9 K. Acknowledgments: FONDECYT 1180939 (UGV), 1171047 (MS-A), FONDECYT postdoctoral 3160803 (MJR), FONDECYT Iniciacion 11160664 (TPC), PhD. scholarship CONICYT (MAC).

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