Dresden 2020 – wissenschaftliches Programm
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BP 9.25: Poster
Montag, 16. März 2020, 17:30–19:30, P2/2OG
Verification of evaporated dry phospholipid bilayer formation with AFM — •Maria Jose Retamal2, Marcelo A. Cisternas1, Francisca Palacios-Coddou1, Sebastian Molina1, Nicolas Moraga1, Hugo Zelada1, Marco A. Soto-Arriaza2, Tomas P. Corrales3, and Ulrich G. Volkmann1 — 1Institute of Physics and CIEN-UC, P. Univ. Catolica de Chile, Santiago, Chile — 2Faculty of Chemistry and CIEN-UC, P. Univ. Catolica de Chile, Santiago, Chile — 3Department of Physics, UTFSM, Valparaiso, Chile
Phospholipids are the main components of the cell membrane, and it is of great interest to study their behavior and phase transitions during temperature changes. In particular, we study bilayer systems on silicon surfaces covered with its native silicon oxide layer (Supported Lipid Bilayers or SLB) using Atomic Force Microscopy in air. For the first time the SLB were formed from their gas phase by a totally dry process and without further hydration. In these SLB it was possible to identify with AFM significant changes in the sample topography at the transition temperatures reported in the literature. The cantilever break through forces for single, double and triple bilayers have been measured. It was also found that the AFM tip is capable to modify at room temperature the -otherwise long time stable- SLB structures. These modifications are directly related to the force exerted by the tip on the SLB. Acknowledgments: FONDECYT 1180939 (UGV), 1171047 (MS-A), FONDECYT postdoctoral 3160803 (MJR), FONDECYT Iniciacion 11160664 (TPC), PhD. scholarship CONICYT (MAC).