Dresden 2020 – wissenschaftliches Programm
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BP 9.8: Poster
Montag, 16. März 2020, 17:30–19:30, P2/2OG
Stochastic modeling of the tug-of-war between kinesin-1 and mammalian dynein motor proteins in intracellular transport — •Gina Antonieta Monzon1, Lara Scharrel2, Ashwin DSouza2, Stefan Diez2,3, and Ludger Santen1 — 1Center for Biophysics, Department of Physics, Saarland University, 66123 Saarbrücken, Germany — 2B CUBE - Center for Molecular Bioengineering, Technische Universität Dresden, 01307 Dresden, Germany — 3Cluster of Excellence Physics of Life, Technische Universität Dresden, 01062 Dresden, Germany
Intracellular transport is a bidirectional, biased stochastic motion performed by teams of kinesin and dynein motors walking in opposite directions along microtubules. A tug-of-war between kinesin and dynein occurs since both motors are involved in the transport. Therfore, it is intringuingly to understand this tug-of-war in order to know how the cell manages targeted cargo transport. In our stochastic kinesin and dynein models  we include all known motor properties and a mechanical dynein activation. Studying bidirectional transport we see a blocked state, where forces between kinesin and dynein are balanced and the motors are strongly localized. Investigating the influence of ATP concentration, we see the blocked state remains stable as long as the forces are constant. Moreover, hindering roadblocks does not influence the blocked state neither because of the motor localization.
: G.A. Monzon, L. Scharrel, L. Santen, S. Diez, Activation of mammalian cytoplasmic dynein in multi-motor motility assays, Journal of Cell Science, 2019.